PhD Scientific Days 2026

Surgical Medicine

The Change of Perfusion in Postoperative Adhesion Formation and the Role of VEGF

Előadó neve

Dr. Zare, Mahan

Neptune code

D6V4UZ

Előadó munkahelye

Faculty of Medicine, Semmelweis University

Előadó telefonszáma

+36706580502

Előadó e-mail címe

Mahan_Zr98@yahoo.com

Az előadás címe

The Change of Perfusion in Postoperative Adhesion Formation and the Role of VEGF

Szerző(k) neve és munkahelye

Dr. Mahan Zare¹

1: Faculty of Medicine, Semmelweis University

Bemutatás módja

Szóbeli

Szekció

Surgical Medicine

Language of the presentation

English

Preferred session

Surgical Medicine

Összefoglaló szövege

Introduction: Postoperative adhesions are abnormal fibrous connections with newly formed vessels within the extracellular matrix. Perfusion is a key determinant of adhesion development, as insufficient blood flow promotes hypoxia, inflammation, and fibrosis. Real-time microvascular assessment with laser speckle contrast imaging (MoorFLPI-2), combined with vascular endothelial growth factor (VEGF), a key angiogenic mediator, enables correlation between hemodynamic changes and molecular drivers during adhesion formation.
Aim: To evaluate the blood flow and VEGF expression dynamics of adhesions in the early postoperative period.
Methods: NMRI mice were anesthetized, and after a midline incision, baseline perfusion was recorded at the adhesion induction site. Scratches were made on the cecum’s surface and peritoneal wall, followed by desiccation. The cecum was repositioned, and a postoperative measurement was taken. Subsequent termination measurements were conducted at 1 h, 2 h, 4 h, 6 h, and at postoperative days 1, 2, 3, 4, 5, and 7 (n = 6 per group). Tissue samples were collected for VEGF quantification by spectrophotometry.
Results: Baseline perfusion averaged 792.8 flux units. Perfusion declined significantly to 339.3 and reached its overall minimum at 4 hours (192.4). From 6 hours onward, perfusion gradually recovered, approached baseline by day 2, peaked on day 5, and decreased again by day 7, forming an early hypoperfusion-recovery-stabilization pattern. VEGF expression showed an initial surge at 2 hours, followed by reduced levels at 4-6 hours and days 2-4, with a secondary rise on day 5 and a slight decline by day 7. Perfusion and VEGF demonstrated a significant inverse correlation (r = - 0.50, p = 0.003), indicating higher VEGF concentrations in more hypoperfused tissue.
Conclusion: This study demonstrates a biphasic perfusion response during early adhesion formation, with an immediate postoperative decline followed by progressive recovery. The rise in perfusion after day 2 suggests ongoing vascular remodeling relevant to adhesion development. VEGF showed an inverse pattern, peaking during early hypoperfusion and later recovery. These coordinated changes highlight time-dependent windows that may guide targeted strategies to prevent adhesions.
Funding: Department of Experimental Cardiology and Surgical Techniques, Semmelweis University

University

Semmelweis University

Supervisor

Dr. Györgyi Szabó

Publication of my abstract

I do not give consent to the publication of my abstract on the website of the congress.

phd.section.field

before finishing undergraduate studies (TDK, MD-PhD)

Kind

Szabad

Status

elfogadva

Accepted presentation method

szóbeli

Előadás fájl jóváhagyás

nem hagyta jóvá

Előadó

9817

Start

17:30

End

17:40