PhD Scientific Days 2026

Surgical Medicine

The Impact of Different Surgical Suture Techniques on Microcirculation in the Postoperative Period

Előadó neve

Dr. Zare, Mahan

Neptune code

D6V4UZ

Előadó munkahelye

Faculty of Medicine, Semmelweis University

Előadó telefonszáma

+36706580502

Előadó e-mail címe

Mahan_Zr98@yahoo.com

Az előadás címe

The Impact of Different Surgical Suture Techniques on Microcirculation in the Postoperative Period

Szerző(k) neve és munkahelye

Dr. Mahan Zare¹

1: Faculty of Medicine, Semmelweis University

Bemutatás módja

Szóbeli

Szekció

Surgical Medicine

Language of the presentation

English

Preferred session

Surgical Medicine

Összefoglaló szövege

Introduction: Optimizing wound closure is critical for postoperative healing; however, real-time effects of different suture techniques on microvascular stability remain poorly defined. Laser Speckle Contrast Imaging (LSCI) is a non-invasive, high-resolution method for quantifying cutaneous blood flow, enabling evaluation of how suturing strategies influence early postoperative (PO) tissue behavior.
Aim: To identify how different suture techniques influence early PO microcirculatory stability.
Methods: Forty Wistar rats (20 males and 20 females) were anesthetized with ketamine–xylazine. Each rat received two 4-cm skin incisions (dorsal and abdominal), yielding 80 wound sites. Ten suture techniques were tested: interrupted (INT)—simple interrupted (SINT), Donati (DO), Allgöwer (AL), X-stitch (XINT), and hidden (HID); and running (RUN)—simple running (SRUN), locked (LRUN), running Donati (DRUN), intracutaneous (IRUN), and running-X (XRUN). Each technique was applied to eight wounds (n=8; equal sex distribution). Perfusion was measured on PO days 1, 2, 3, 4, 6, and 10.
Results: INT sutures showed greater variability in PO perfusion than RUN techniques. Within the INT group, DO and XINT exhibited early–mid PO increases followed by declines, whereas AL showed a more gradual and sustained increase. SINT and HID displayed moderate and stable perfusion recovery. Among RUN sutures, DRUN and XRUN showed mid-PO perfusion peaks with subsequent declines, while SRUN and IRUN exhibited smoother perfusion trajectories. Kruskal–Wallis analysis revealed significant time-dependent changes for DO and XINT (p < 0.05), whereas no significant temporal changes were detected for the remaining techniques (p > 0.05). No significant sex-related differences were observed.
Conclusion: LSCI effectively differentiates the microcirculatory responses caused by a wide range of suture architectures, demonstrating that stitch geometry and tension distribution play a key role in shaping PO vascular behavior. Techniques that distribute tension more uniformly produced more stable microvascular trajectories, whereas methods that apply greater edge compression exhibited marked fluctuations. These findings may provide objective guidance for selecting suture methods that optimize PO tissue perfusion.
Funding: Department of Experimental Cardiology and Surgical Techniques, Semmelweis University

University

Semmelweis University

Supervisor

Dr. Györgyi Szabó, Dr. Csukás Domokos

Publication of my abstract

I do not give consent to the publication of my abstract on the website of the congress.

phd.section.field

before finishing undergraduate studies (TDK, MD-PhD)

Kind

Szabad

Status

elfogadva

Accepted presentation method

szóbeli

Előadás fájl jóváhagyás

nem hagyta jóvá

Előadó

9817

Start

17:45

End

17:55