PhD Scientific Days 2026

Pharmaceutical Sciences and Health Technologies 4.

Storage Conditions and Cryoprotectants Influence the Stability of Extracellular Vesicles

Előadó neve

Ms. Nagy, Mátka

Neptune code

G0SOAU

Előadó munkahelye

Department of Physiology, Semmelweis University, Budapest, Hungary

Előadó telefonszáma

+36307790850

Előadó e-mail címe

nagy.matka@gmail.com

Az előadás címe

Storage Conditions and Cryoprotectants Influence the Stability of Extracellular Vesicles

Szerző(k) neve és munkahelye

Mátka Nagy¹, Balázs Kondoros², Tamás Kiss², Tamás Sohajda², Ákos M. Lőrincz^1,3

1: Department of Physiology, Semmelweis University, Budapest, Hungary
2: CarboHyde Zrt, Budapest, Hungary
3: Second Department of Internal Medicine, Szent György Hospital, Székesfehérvár, Hungary

Bemutatás módja

Szóbeli

Szekció

Pharmaceutical Sciences and Health Technologies 4.

Language of the presentation

English

Preferred session

Pharmaceutical Sciences and Health Technologies

Összefoglaló szövege

Introduction
The storage of extracellular vesicles (EVs) remains a critical challenge, as environmental conditions may compromise their integrity, content, and function. Owing to their nanoscale size and structural fragility, optimized storage protocols are essential for reproducible research outcomes and future clinical applications. Based on previous results from our workgroup, storage at −80 °C provides better preservation than storage at room temperature, 4 °C or −20 °C.

Aim
Our aim was to improve EV storage at −80 °C by examining different physicochemical conditions and the effects of various cryoprotectants.

Methods
Neutrophil-derived EVs were generated and stored either at −80 °C or lyophilized and kept for one month. Carbohydrates were used as cryoprotectants during storage. Particle counts were determined by flow cytometry, size distribution by nanoparticle tracking analysis, membrane integrity by lactate dehydrogenase (LDH) release assay, and morphology by transmission electron microscopy.

Results
Compared with fresh controls, particle counts were reduced under all storage conditions, including storage at −80 °C and lyophilization. In lyophilized samples, cryoprotectants reduced this loss, as shown by significantly higher event counts. This cryoprotective effect was particularly evident when carbohydrate-based cryoprotectants were applied, resulting not only in higher vesicle counts but also in better preservation of membrane integrity. LDH release was consistently lower, suggesting reduced membrane disruption.

Conclusion
This study provides a comparative evaluation of EV storage conditions, highlighting the beneficial role of cryoprotectants in maintaining vesicle stability. Our findings support the use of carbohydrate-based cryoprotectants as effective approaches for preserving both EV quantity and integrity.

Funding
EFOP-3.6.3-VEKOP-16-2017-00009, NKFIH FK 137770, TKP2021-EGA-24
MSCA SE: Bicyclos
2024-2.1.2-EKÖP-KDP-2024-00002

University

Semmelweis University

Supervisor

Dr. Ákos M. Lőrincz

Publication of my abstract

I do not give consent to the publication of my abstract on the website of the congress.

phd.section.field

in doctoral studies after complex exam (PhD)

Kind

Szabad

Status

elfogadva

Accepted presentation method

szóbeli

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

8248

Start

17:30

End

17:40