PhD Scientific Days 2026

Molecular Medicine 4.

Prediction of Implantation Success Using a Complex Animal Model for Embryo-Endometrial Crosstalk

Előadó neve

Mr. Nagy, Bence

Neptune code

CC58NV

Előadó munkahelye

Institute of Genetics, Cell- and Immunbiology

Előadó telefonszáma

06207705375

Előadó e-mail címe

nagy.bencep94@gmail.com

Az előadás címe

Prediction of Implantation Success Using a Complex Animal Model for Embryo-Endometrial Crosstalk

Szerző(k) neve és munkahelye

Bence Nagy¹, Zoltán Bognár², Timea Judit Csabai², Gergely Berta², Nóra Fekete¹, Éva Pállinger¹

1: Institute of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary
2: Department of Medical Biology, Medical School, University of Pécs, Pécs, Hungary

Bemutatás módja

Szóbeli

Szekció

Molecular Medicine 4.

Language of the presentation

English

Preferred session

Molecular Medicine

Összefoglaló szövege

Introduction
The efficacy of assisted reproductive technologies is significantly affected by the timing of embryo transfer. However, there is no widely accepted biomarker that can identify the window of implantation. Deviating by 12 hours from the optimal transfer time decreases the likelihood of successful implantation by around 45%.
Aims
This study aims to conduct a comprehensive molecular analysis of embryo-endometrium communication, focusing on soluble mediators derived from the endometrium, as well as extracellular vesicle (EV) patterns and the implantation-associated transcriptome.
Methods
A murine animal model was used to characterise the pre-receptive and receptive phases of the endometrium. Samples were collected at 2.5 and 4.5 days post-coitum (dpc). Serum progesterone levels and endometrial progesterone receptor expression were assessed. Uterine lavage was used to analyse cytokines and extracellular vesicles (EVs). The expression levels of implantation-associated adhesion molecules were determined by RT-PCR.
Results
The investigated analytes can differentiate between the pre-receptive and receptive phases of the endometrium. At 2.5 dpc, significantly higher amounts of EVs expressing CD24, CD29, CD326, CD44, CD49b, CD49a and CD66 could be determined. In contrast, at 4.5 dpc, the concentrations of proinflammatory IL-6, IFN-γ and MCP-1 significantly decreased, while CD44, CD81 and CD29 were significantly upregulated.
Conclusion
These findings demonstrate clear molecular differences between receptive and non-receptive phases, suggesting that uterine lavage analysis may provide a basis for identifying noninvasive biomarkers of implantation in humans.
Funding
This work was supported by the National Laboratory on Human Reproduction. Project no. RRF-2.3.1-21-2022-00012, titled "National Laboratory on Human Reproduction," was conducted with backing from the European Union's Recovery and Resilience Facility under the Széchenyi Plan Plus Program. The project received funding from the HUN-REN Hungarian Research Network and STIA_2024 (19279/PMKP/2025). Project no. TKP-2021-EGA-10 was executed with support from the National Research, Development, and Innovation Fund of Hungary, which was funded under the TKP-2021-EGA program. The PTE Medical School (Szolcsányi Foundation) supported the work.

University

Semmelweis University

Supervisor

Dr. Pállinger Éva

Publication of my abstract

I give consent to the publication of my abstract on the website of the congress.

phd.section.field

in doctoral studies after complex exam (PhD)

Kind

Szabad

Status

elfogadva

Accepted presentation method

szóbeli

Előadás fájl jóváhagyás

jóváhagyta

Előadó

7477

Start

11:15

End

11:25