PhD Scientific Days 2026

Poster Session 3.R - Cardiovascular Medicine and Research

2D and 3D Microcarrier Grown Mesenchymal Stem Cell Derived Extracellular Vesicles Comparison, Functional Study and their Utilization for the Reduction of Cchemotheraputics-caused Cardiotoxicity

Előadó neve

Rácz, Richárd, MSc

Neptune code

etgd69

Előadó munkahelye

Semmelweis University, Faculty of Medicine, Department of Pharmacology and Pharmacotherapy

Előadó telefonszáma

06304674552

Előadó e-mail címe

racz2.richard4@gmail.com

Az előadás címe

2D and 3D Microcarrier Grown Mesenchymal Stem Cell Derived Extracellular Vesicles Comparison, Functional Study and their Utilization for the Reduction of Cchemotheraputics-caused Cardiotoxicity

Szerző(k) neve és munkahelye

Rácz Richárd¹, Hambalkó Szabolcs¹, Dr. Giricz Zoltán¹, Wagner Nóra¹

1: Semmelweis University, Faculty of Medicine, Department of Pharmacology and Pharmacotherapy

Bemutatás módja

Poszter

Szekció

Poster Session 3.R - Cardiovascular Medicine and Research

Language of the presentation

English

Preferred session

Cardiovascular Medicine and Research

Összefoglaló szövege

Chemotherapeutic agents are widely used in the treatment of various cancers. However, their cardiotoxic side effects limit their clinical efficacy and compromise patient health. These drugs induce oxidative stress, inflammation, and cellular apoptosis in cardiac tissues, leading to cardiomyopathy, arrhythmias, and heart failure. As a result, strategies to mitigate these adverse effects are urgently needed to improve the quality of life and survival rates of cancer patients.
Mesenchymal stem cells (MSCs) have gained significant attention for their regenerative potential, partly attributed to their secretion of extracellular vesicles (EVs). MSC-derived EVs carry bioactive molecules, such as proteins, lipids, and RNAs, that can modulate cellular behaviour, promote tissue repair, and reduce inflammation. Recent studies have demonstrated the cardioprotective properties of MSC EVs.
Mesenchymal stem cells are exclusively adherent; they can proliferate only once attached to a surface. For this reason, they can grow in 2D flask-based environments, 3D microcarrier-based spinner flasks, or bioreactors. 3D microcarrier systems allow for increased cell proliferation and improved EV release while still potentially maintaining the quality and functionality of the 2D systems.
We aim to validate and understand the therapeutic potential and differences between 2D and 3D MSC EVs in counteracting the cardiotoxicity induced by doxorubicin, 5-fluorouracil, trastuzumab, and lapatinib. Specifically, we explore the molecular mechanisms by which MSC EVs exert their protective effects, including modulation of oxidative stress, inhibition of apoptosis, changes in mitophagy and autophagy, and promotion of cardiac tissue regeneration while also measuring and comparing the health of the MSCs, their proliferation, EV production and the quality of their EVs.
In conclusion, MSC-derived EVs represent a promising therapeutic strategy for reducing the cardiotoxic side effects of chemotherapeutics. Utilising the advantages of the 3D microcarrier-based systems, we can improve the proliferation of our MSC cells, increase their EV production, while the EVs still maintain their quality and functionality. By harnessing the natural regenerative properties of MSC EVs, it may be possible to enhance the safety and efficacy of cancer treatments, providing a vital improvement in patient outcomes.

University

Semmelweis University

Supervisor

Dr. Giricz Zoltán

Publication of my abstract

I do not give consent to the publication of my abstract on the website of the congress.

phd.section.field

in doctoral studies before complex exam (PhD)

Kind

Szabad

Status

elfogadva

Accepted presentation method

poszter

Előadás fájl jóváhagyás

nem rendelkezett róla

Előadó

9121

Start

14:18

End

14:21